Supplemental Data Core Transcriptional Regulatory Circuitry in Human Embryonic Stem Cells
نویسندگان
چکیده
Supplemental Text Human ES cell controls Immunohistochemical Analysis of Pluripotency Markers Teratoma Formation Embryoid Bodies Chromatin Immunoprecipitation Assay Antibodies Location Analysis Protocols Control ChIP experiments Array Design Selection of Regions and Design of Subsequences Compiled Probes and Controls Replicate Data Sets Array Scanning and Data Extraction Data Normalization and Analysis Identification of Bound Regions Comparing Transcription Factor Bound Regions to Known Genes Comparisons to Known Genes Analysis of Error Rates in Location Analysis Expression Analysis Gene Ontology Classification Oct4, Sox2 and Nanog Binding to the Oct4 Promoter Proximal Region Oct4 and Sox2 Binding to UTF1 and FGF4 Distribution of Oct4, Sox2, and Nanog Binding Sites Relative to Transcription Start Sites
منابع مشابه
Models of human core transcriptional regulatory circuitries.
A small set of core transcription factors (TFs) dominates control of the gene expression program in embryonic stem cells and other well-studied cellular models. These core TFs collectively regulate their own gene expression, thus forming an interconnected auto-regulatory loop that can be considered the core transcriptional regulatory circuitry (CRC) for that cell type. There is limited knowledg...
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It has been known that three core transcription factors (TFs), NANOG, OCT4, and SOX2, collaborate to form a transcriptional circuitry to regulate pluripotency and self-renewal of human embryonic stem (ES) cells. Similarly, MYC also plays an important role in regulating pluripotency and self-renewal of human ES cells. However, the precise mechanism by which the transcriptional regulatory network...
متن کاملCore Transcriptional Regulatory Circuitry in Human Embryonic Stem Cells
The transcription factors OCT4, SOX2, and NANOG have essential roles in early development and are required for the propagation of undifferentiated embryonic stem (ES) cells in culture. To gain insights into transcriptional regulation of human ES cells, we have identified OCT4, SOX2, and NANOG target genes using genome-scale location analysis. We found, surprisingly, that OCT4, SOX2, and NANOG c...
متن کاملA regulatory circuitry comprised of miR-302 and the transcription factors OCT4 and NR2F2 regulates human embryonic stem cell differentiation.
Multiple levels of control are in play to regulate pluripotency and differentiation in human embryonic stem cells (hESCs). At the transcriptional level, the core factors OCT4, NANOG and SOX2 form a positive autoregulatory loop that is pivotal for maintaining the undifferentiated state. At the post-transcriptional level, microRNAs (miRNAs) belonging to the miR-302 family are emerging as key play...
متن کاملdbCoRC: a database of core transcriptional regulatory circuitries modeled by H3K27ac ChIP-seq signals
Core transcription regulatory circuitry (CRC) is comprised of a small group of self-regulated transcription factors (TFs) and their interconnected regulatory loops. Studies from embryonic stem cells and other cellular models have revealed the elementary roles of CRCs in transcriptional control of cell identity and cellular fate. Systematic identification and subsequent archiving of CRCs across ...
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